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Upgrades to Panorama

The latest set of upgrades to Panorama allows us to eliminate the sequencing of the buffy coat from the maternal blood sample, as well as to eliminate the paternal buccal swab. These changes are expected to improve the ease of use for clinics while maintaining the same accuracy.

Test performance was evaluated in an internal validation study demonstrating similar accuracy.

Comparison of Panorama performance in validation studies

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Frequently Asked Questions

1. Can Panorama® still distinguish between mom and baby?
Yes, because Panorama utilizes SNPs, we are able to infer maternal and fetal signals using the plasma alone and do not require the buffy coat to do so.
2. Is there a change to turnaround time?
No, turnaround time will remain the same.
3. Will the accuracy of Panorama change?
No, the updated version will result in the same accuracy you have come to expect, which was established in Panorama’s validation study.1

1Pergament E et al. Single-nucleotide polymorphism-based noninvasive prenatal screening in a high-risk and low-risk cohort. Obstet & Gynecol 2014; 124(2 Pt 1): 210-218.

4. Will microdeletions be affected by these changes?
No, there is no change to the microdeletion portion of Panorama.
5. What about patients with a high BMI? I used to send in a paternal cheek swab to increase the likelihood of getting a result.
The improvements to Panorama allow us to eliminate the paternal cheek swab without increasing the redraw rate for these cases.
6. How do you calculate fetal fraction now without the buffy coat?
Panorama will continue to use the SNP-based methodology to determine fetal fraction from the plasma.
7. Is the sample size in the internal validation large enough?
Internal validation was designed to prove that test accuracy is no different from the prior version. The sensitivity/specificity always are bounded by confidence intervals, and these intervals are actually smaller then observed with previous version.
8. Will Panorama still be able to call vanishing twin, triploidy, and molar pregnancy?
Yes. The elimination of the buffy coat and paternal sample has no effect on our ability to recognize different "DNA signatures", or haplotypes, in a blood sample.
9. Can Panorama still identify maternal mosaicism?
In cases of maternal mosaicism (with the exception of rare cases when the percent of mosaicism in the matenal blood is identical to the fetal fraction), the allele frequencies that are identified will allow us to distinguish between uninformative SNP patterns in the mother and fetus. These "noisy" SNP patterns will still result in a "no call." As with any molecular technology there are cases of mosaicism that are below the limit for the analysis to identify.